Colorimetric determination of tyrosine

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Colorimetric Determination of Tyrosine BY G. CERIOTTI AND L. SPANDRIO

The procedure described permits quantitative application of the Sakaguchi reaction. The greatest advantage of the method is the stability of the coloured product in butanol. The sensitivity, already high in the original method, is further increased, since the volume of the final coloured solution is reduced by the extraction. For concentrations of arginine ranging between 0-5 and 6 ,ug./ml. the...

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Colorimetric Determination of Serum

In the past few years there has been renewed interest in the study of serum cholinesterase in various disease states, particularly those affecting the liver. Recently, a colorimetric method for the determination of this enzyme in the serum was reported by Ravin, Tsou, and Seligman (1). Their method, simple to perform, is as accurate as the gastrometric technique and more specific than the elect...

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Colorimetric determination of blood arginase.

N EXTENSIVE LITERATURE Ofl arginase activity in tissues and tissue homogenates has accumulated in recent years. Roberts (1) has studied the arginase activity of liver, kidney, and epidermis of the Swiss mouse and of a transplantable squamous cell carcinoma. He found that liver had the greatest activity, followed by carcinoma, epidermis, and kidney. Sano (2) isolated an enzyme from rabbit intest...

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Colorimetric determination of blood lipides.

Extraction-As the oxidation is essentially non-specific, the plasma extract must be as free of non-lipide reducing substances as possible. All solvents should be tested occasionally for reducing residues remaining after evaporation, and redistilled if necessary. Ether should be maintained peroxide-free. The determinations reported here followed a conventional alcohol-ether extraction and subseq...

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Quantitative colorimetric determination of tryptophan.

The methods currently used for the estimation of tryptophan are based on one of the following principles: Reaction with aldehydes in acid solution (1, Z), with glyoxylic acid (3), with mercury salts and nitrous acid (4,5), by determination after isolation from its mercury salt (6)) or by the use of Marshal’s reagent (7). Of the above mentioned methods, those based on aldehyde reactions and on t...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1957

ISSN: 0306-3283

DOI: 10.1042/bj0660607